P-14: The Effects of Depression on Reduced Sperm Motility in Male Rats

Background: Infertility is a problem for 15 to 30% couples. Male infertility may be a result of insufficient number of sperms and poor motility. Spermatozoon consists of a head,midpiece and tail. The long tail consist micro-tubules that from part of the propulsion system of the spermatozoon. Motility of sperm is the result of relation between ATP,micro-tubules and dynein. Depression is one of the most prevalent and disabling disorders,in the world. Depression can effect in fertilization by decreasing motility of sperm. Study there is any sperm motility dysfunction in stress induced depression. Materials and Methods: 40 male sprague dawley rats entered the study.Half of them had unpredictable chronic mild stress(UCMS)for 21 days in order to get depressed. Forced swim test(FST) showed the immobility time(IB), the symptom of depression. One week after behavioral test, rats were prepared for transcardial perfusion. Then after paraffin, fixed brain was taken off and hippocampus was prepared for nissl staining. Fresh epididym were taken off and got homogenized and the supranatant was reserved for Real Time PCR evaluation. This evaluation how the level of dynein experssion.All above procedures were performed for control group too,instead of inducing UCMS. Results: Our results showed low weigh gain and increased IB in UCMS groups(p<0.05). There was a higher concentration of serum IL6 in depressed groups in comparison to control one(p<0.05). Nissle staining showed a prominent neural degeneration in UCMS group hippocampus(p<0.05). At last RT PCR showed a lower permission of dynein gene in epididym (p<0.05). Conclusion: Our studies showed different manifestations of depression after UCMS. It showed that UCMS can lead to mental depression. RT-PCR studies showed low experssion of dynein (p<0.05), which is equal to impairment of sperm motility. In this study we showed that depression can reduce sperm motility by low expression of dynein that existed in tail of sperm.